Article
Sequence-specific "gene signatures" can be obtained by PCR with single specific primers at low stringency.
Proceedings of the National Academy of Sciences of the United States of America - 1 Mar 1994
Pena S D, Barreto G, Vago A R, De Marco L, Reinach F C, Dias Neto E, Simpson A J
Abstract excerpt
Low-stringency single specific primer PCR (LSSP-PCR) is an extremely simple PCR-based technique that detects single or multiple mutations in gene-sized DNA fragments. A purified DNA fragment is subjected to PCR using high concentrations of a single specific oligonucleotide primer, large amounts o...
Topics
- Animals
- Base Sequence
- Chickens
- DNA Mutational Analysis
- DNA Primers
- DNA, Mitochondrial
- Diabetes Insipidus
- Female
- Genetic Linkage
- Genetic Variation
- Humans
- Male
- Molecular Sequence Data
- Myosins
- Polymerase Chain Reaction