Article
Sequence-specific "gene signatures" can be obtained by PCR with single specific primers at low stringency.
Proceedings of the National Academy of Sciences of the United States of America - 1 Mar 1994
Pena S D, Barreto G, Vago A R, De Marco L, Reinach F C, Dias Neto E, Simpson A J
Abstract excerpt
Low-stringency single specific primer PCR (LSSP-PCR) is an extremely simple PCR-based technique that detects single or multiple mutations in gene-sized DNA fragments. A purified DNA fragment is subjected to PCR using high concentrations of a single specific oligonucleotide primer, large amounts of Taq polymerase, and a very low annealing temperature. Under these conditions the primer hybridizes specifically to...
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