Temperature-sensitive mutants and revertants in the coronavirus nonstructural protein 5 protease (3CLpro) define residues involved in long-distance communication and regulation of protease activity.

Abstract excerpt

Positive-strand RNA virus genomes are translated into polyproteins that are processed by viral proteases to yield functional intermediate and mature proteins. Coronaviruses (CoVs) carry genes that encode an nsp5 protease (also known as 3CLpro or Mpro) responsible for 11 maturation cleavages. The nsp5 structure contains two chymotrypsin-like domains (D1 and D2) and a unique domain (D3), and forms functional...

Topics

• Alleles
• Amino Acid Sequence
• Amino Acid Substitution
• Animals
• Coronavirus 3C Proteases
• Cricetinae
• Cysteine Endopeptidases
• Enzyme Activation
• Gene Order
• Mice
• Models, Molecular
• Molecular Sequence Data
• Murine hepatitis virus