Article
Expression, purification, crystallization, and preliminary x-ray analysis of casein kinase-1 from Schizosaccharomyces pombe.
The Journal of biological chemistry - 11 Mar 1994
Carmel G, Leichus B, Cheng X, Patterson S D, Mirza U, Chait B T, Kuret J
Abstract excerpt
The catalytic domain of Schizosaccharomyces pombe casein kinase-1 (the product of the cki1 gene) has been overexpressed in Escherichia coli, purified by chromatographic methods, characterized in vitro, and crystallized in the presence and absence of nucleotide substrate. The best crystals belong...
Topics
- Amino Acid Sequence
- Casein Kinases
- Catalysis
- Chromatography, Ion Exchange
- Cloning, Molecular
- Crystallography, X-Ray
- Escherichia coli
- Kinetics
- Mass Spectrometry
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Protein Kinases
- Schizosaccharomyces
- Sequence Deletion