Article
Cloning and disruption of CKB1, the gene encoding the 38-kDa beta subunit of Saccharomyces cerevisiae casein kinase II (CKII). Deletion of CKII regulatory subunits elicits a salt-sensitive phenotype.
The Journal of biological chemistry - 5 May 1995
Bidwai A P, Reed J C, Glover C V
Abstract excerpt
Saccharomyces cerevisiae casein kinase II (CKII) contains two distinct catalytic (alpha and alpha') and regulatory (beta and beta') subunits. We report here the isolation and disruption of the gene, CKB1, encoding the 38-kDa beta subunit. The predicted Ckb1 sequence includes the N-terminal autophosphorylation site, internal acidic domain, and potential metal binding motif (CPX3C-X22-CPXC) present in other beta...
Topics
- Amino Acid Sequence
- Antisense Elements (Genetics)
- Base Sequence
- Casein Kinase II
- Cations, Monovalent
- Chromosome Mapping
- Cloning, Molecular
- DNA Primers
- Genes, Fungal
- Molecular Sequence Data