Article
Stabilization in microsomal membranes of the fifth transmembrane segment of the Na+,K(+)-ATPase alpha subunit with proline to leucine mutation.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Jan 2000
Homareda H, Kawakami K, Nagano K, Matsui H
Abstract excerpt
We have reported that the fifth transmembrane segment of the Na+,K(+)-ATPase alpha subunit could not be inserted into microsomal membranes when it was synthesized from the truncated alpha 1 cDNA. In this study, three proline residues in the segment were changed into leucine residues by site-directed mutagenesis. Replacement of all three proline residues by leucine residues allowed the insertion of this segment...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Dogs
- Enzyme Stability
- Glycosylation
- Intracellular Membranes
- Leucine
- Microsomes
- Molecular Sequence Data