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Article

Stabilization in microsomal membranes of the fifth transmembrane segment of the Na+,K(+)-ATPase alpha subunit with proline to leucine mutation.

Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Jan 2000

Homareda H, Kawakami K, Nagano K, Matsui H

Abstract excerpt

We have reported that the fifth transmembrane segment of the Na+,K(+)-ATPase alpha subunit could not be inserted into microsomal membranes when it was synthesized from the truncated alpha 1 cDNA. In this study, three proline residues in the segment were changed into leucine residues by site-directed mutagenesis. Replacement of all three proline residues by leucine residues allowed the insertion of this segment...

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PMID: 8123258
DOI: 10.1139/o93-060

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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