DNaseI footprinting studies of Escherichia coli biotin repressor-operator interactions.

doi:10.1093/oxfordjournals.jbchem.a124235

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Article

DNaseI footprinting studies of Escherichia coli biotin repressor-operator interactions.

Journal of biochemistry - 1 Nov 1993

Lin K C, Shiuan D

Abstract excerpt

Transcription of Escherichia coli biotin operon is repressed by biotin repressor in the presence of biotinyl-5'-adenylate as a corepressor. To determine precisely the site of action of biotin repressor on the operator sequence, DNaseI footprinting experiments were performed on the PCR-produced bi...

Topics

Adenosine Monophosphate
Base Sequence
Biotin
Densitometry
Deoxyribonuclease I
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Molecular Sequence Data
Mutation
Operator Regions, Genetic
Polymerase Chain Reaction
Repressor Proteins
Sequence Alignment
Transcription, Genetic

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