Article
The activities of recombinant gamma-carboxyglutamic-acid-deficient mutants of activated human protein C toward human coagulation factor Va and factor VIII in purified systems and in plasma.
Biochemistry - 22 Feb 1994
Jhingan A, Zhang L, Christiansen W T, Castellino F J
Abstract excerpt
The dependence of the activity of recombinant activated human protein C (r-APC) on each of its nine gamma-carboxyglutamic (Gla) residues (sequence positions 6, 7, 14, 16, 19, 20, 25, 26, and 29) has been assessed in purified systems and in plasma using r-mutants in which each Gla residue of r-APC was individually altered to an Asp (D) residue. The assays employed included a factor Va inactivation assay in the...
Topics
- 1-Carboxyglutamic Acid
- Calcium
- Factor VIII
- Factor Va
- Humans
- Kinetics
- Mutation
- Partial Thromboplastin Time
- Protein C
- Prothrombin Time
- Recombinant Proteins