Article
Identification of germ-line chimaeras by polymerase chain reaction and isoenzyme analysis of mouse spermatozoa.
Journal of reproduction and fertility - 1 Nov 1993
Mann G B, Fowler K J, Grail D, Dunn A R
Abstract excerpt
In this study a rapid, simple and inexpensive procedure is described which allows potential germ-line male mice to be identified with confidence. Spermatozoa recovered by uterine washing following mating with normal female mice was analysed in two ways. First, the patterns of expression of the different isoforms of glucose phosphate isomerase were determined. Since the glucose phosphate isomerase isoforms...
Topics
- Animals
- Base Sequence
- Cells, Cultured
- Chimera
- DNA
- Glucose-6-Phosphate Isomerase
- Isoenzymes
- Male
- Mice
- Mice, Inbred Strains
- Mice, Transgenic
- Molecular Sequence Data