Article
Metabolic responses to substrate futile cycling in Escherichia coli.
The Journal of biological chemistry - 18 Feb 1994
Chao Y P, Liao J C
Abstract excerpt
A cyclic pathway between phosphoenolpyruvate and oxaloacetate was created in Escherichia coli by simultaneous overexpression of phosphoenolpyruvate carboxykinase (encoded by pck) and phosphoenopyruvate carboxylase (encoded by ppc) from a multicopy plasmid under the control of the tac promoter. Th...
Topics
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Genotype
- Glucose
- Isopropyl Thiogalactoside
- Kinetics
- Models, Biological
- Oxaloacetates
- Oxygen Consumption
- Phosphoenolpyruvate
- Phosphoenolpyruvate Carboxykinase (GTP)
- Phosphoenolpyruvate Carboxylase
- Plasmids
- Species Specificity
