Article
X-Neu5Ac: a novel substrate for chromogenic assay of neuraminidase activity in bacterial expression systems.
Bioorganic & medicinal chemistry - 1 Aug 1993
Fujii I, Iwabuchi Y, Teshima T, Shiba T, Kikuchi M
Abstract excerpt
A chromogenic substrate 1, 5-bromo-4-chloroindol-3-yl 5-acetamido-3,5-dideoxy-alpha-D-glycero-D-galacto-2-nonulopyranosidon ic acid (X-Neu5Ac), has been synthesized to facilitate the screening of bacterial colonies or plaques for the detection of either natural or mutant neuraminidase activity. Substrate 1 was hydrolyzed by neuraminidase isolated from Clostridium perfringens to release a halogenated indol-3-ol 2...
Topics
- Chromogenic Compounds
- Clostridium perfringens
- Hydrolysis
- Indoles
- Magnetic Resonance Spectroscopy
- Mutation
- Neuraminic Acids
- Neuraminidase
- Oxidation-Reduction
- Spectrophotometry, Ultraviolet
- Substrate Specificity