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Directed evolution of D-sialic acid aldolase to L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase.

Proceedings of the National Academy of Sciences of the United States of America - 28 Jun 2005

Hsu Che-Chang, Hong Zhangyong, Wada Masaru, Franke Dirk, Wong Chi-Huey

Abstract excerpt

An efficient L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase was created by directed evolution from the Escherichia coli D-Neu5Ac (N-acetylneuraminic acid, D-sialic acid) aldolase. Five rounds of error-prone PCR and iterative screening were performed with sampling of 10(3) colonies per round. The specificity constant (kcat/Km) of the unnatural sugar L-KDO is improved to a level equivalent to the wild-type...

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Identifiers

PMID: 15967977
DOI: 10.1073/pnas.0504033102
PMCID: PMC1166642

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