Article
Contribution of residue 192 in factor Xa to enzyme specificity and function.
The Journal of biological chemistry - 7 Jul 1995
Rezaie A R, Esmon C T
Abstract excerpt
Mutation of residue 192 (chymotrypsin numbering) from Glu to Gln in thrombin and activated protein C has been shown to dramatically alter substrate and inhibitor specificity, in large part by allowing these enzymes to accept substrates with acidic residues in the P3 and/or P3' positions. In factor Xa, residue 192 is already a Gln. We now compare the properties of a Q192E mutant of Gla-domainless factor X (GDFX)....
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Enzyme Activation
- Enzyme Precursors
- Factor Va
- Factor X
- Factor Xa
- Factor Xa Inhibitors
- Glutamine
- Humans
- Lipoproteins