Article
Zymogenic and enzymatic properties of the 70-80 loop mutants of factor X/Xa.
Protein science : a publication of the Protein Society - 1 Feb 2004
Chen Lin, Manithody Chandrashekhara, Yang Likui, Rezaie Alireza R
Abstract excerpt
The Ca(2+) binding 70-80 loop of factor X (fX) contains one basic (Arg(71)) and three acidic (Glu(74), Glu(76), and Glu(77)) residues whose contributions to the zymogenic and enzymatic properties of the protein have not been evaluated. We prepared four Ala substitution mutants of fX (R71A, E74A, E76A, and E77A) and characterized their activation kinetics by the factor VIIa and factor IXa in both the absence and...
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