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Analysis of yeast DNA topoisomerase II mutants resistant to the antitumor drug amsacrine.

Cancer research - 1 Apr 1994

Wasserman R A, Wang J C

Abstract excerpt

Site-directed mutagenesis of regions within a plasmid-borne yeast TOP2 gene encoding DNA topoisomerase II and hydroxylamine mutagenesis of the entire plasmid were carried out, and the mutagenized plasmid DNA pools were used separately to transform yeast with a temperature-sensitive top2-4 mutation in the chromosomal TOP2 locus. By selecting transformants that grow in the presence of the antitumor drug amsacrine...

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PMID: 8137294

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Analysis of yeast DNA topoisomerase II mutants resistant to the antitumor drug amsacrine.PMID 8137294Read ↗

Mechanistic studies of amsacrine-resistant derivatives of DNA topoisomerase II. Implications in resistance to multiple antitumor drugs targeting the enzyme.Semantic proximity Read ↗

Using yeast to study resistance to topoisomerase II-targeting drugs.Semantic proximity Read ↗

Yeast topoisomerase II mutants resistant to anti-topoisomerase agents: identification and characterization of new yeast topoisomerase II mutants selected for resistance to etoposide.Semantic proximity Read ↗

The top2-5 mutant of yeast topoisomerase II encodes an enzyme resistant to etoposide and amsacrine.Semantic proximity Read ↗

Amsacrine and etoposide hypersensitivity of yeast cells overexpressing DNA topoisomerase II.Semantic proximity Read ↗

Complementation of temperature-sensitive topoisomerase II mutations in Saccharomyces cerevisiae by a human TOP2 beta construct allows the study of topoisomerase II beta inhibitors in yeast.Semantic proximity Read ↗

A temperature sensitive topoisomerase II allele confers temperature dependent drug resistance on amsacrine and etoposide: a genetic system for determining the targets of topoisomerase II inhibitors.Semantic proximity Read ↗

Functional expression of human topoisomerase II alpha in yeast: mutations at amino acids 450 or 803 of topoisomerase II alpha result in enzymes that can confer resistance to anti-topoisomerase II agents.Semantic proximity Read ↗

Isolation and characterization of mAMSA-hypersensitive mutants. Cytotoxicity of Top2 covalent complexes containing DNA single strand breaks.Semantic proximity Read ↗

Mutations in the gyrB domain of eukaryotic topoisomerase II can lead to partially dominant resistance to etoposide and amsacrine.Semantic proximity Read ↗

Identification of yeast DNA topoisomerase II mutants resistant to the antitumor drug doxorubicin: implications for the mechanisms of doxorubicin action and cytotoxicity.Semantic proximity Read ↗

Mutation E522K in human DNA topoisomerase IIbeta confers resistance to methyl N-(4'-(9-acridinylamino)-phenyl)carbamate hydrochloride and methyl N-(4'-(9-acridinylamino)-3-methoxy-phenyl) methane sulfonamide but hypersensitivity to etoposide.Semantic proximity Read ↗

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