Structural studies on peptides corresponding to mutants of the major alpha-helix of barnase.

doi:10.1021/bi00199a027

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Article

Structural studies on peptides corresponding to mutants of the major alpha-helix of barnase.

Biochemistry - 23 Aug 1994

Kippen A D, Arcus V L, Fersht A R

Abstract excerpt

The structures of a family of peptides that contain variants of the major alpha-helix of barnase (residues 6-18) have been analyzed in solution by circular dichroism (CD) and 1H NMR at various concentrations of the helix-inducing cosolvent trifluoroethanol (TFE). The very low equilibrium constant...

Topics

Amino Acid Sequence
Bacterial Proteins
Circular Dichroism
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Mutation
Peptide Fragments
Protein Structure, Secondary
Ribonucleases
Thermodynamics
Trifluoroethanol
Water

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