Article
Transformation of Drosophila melanogaster with the wild-type myosin heavy-chain gene: rescue of mutant phenotypes and analysis of defects caused by overexpression.
The Journal of cell biology - 1 Aug 1994
Cripps R M, Becker K D, Mardahl M, Kronert W A, Hodges D, Bernstein S I
Abstract excerpt
We have transformed Drosophila melanogaster with a genomic construct containing the entire wild-type myosin heavy-chain gene, Mhc, together with approximately 9 kb of flanking DNA on each side. Three independent lines stably express myosin heavy-chain protein (MHC) at approximately wild-type leve...
Topics
- Alleles
- Animals
- Base Sequence
- Cloning, Molecular
- DNA
- DNA Transposable Elements
- Drosophila melanogaster
- Female
- Gene Expression Regulation
- Male
- Molecular Sequence Data
- Mutation
- Myosins
- Phenotype
- Transformation, Genetic