Article
Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants.
The Journal of cell biology - 1 Aug 1994
Kadowaki T, Chen S, Hitomi M, Jacobs E, Kumagai C, Liang S, Schneiter R, Singleton D, Wisniewska J, Tartakoff A M
Abstract excerpt
To understand the mechanisms of mRNA transport in eukaryotes, we have isolated Saccharomyces cerevisiae temperature-sensitive (ts) mutants which accumulate poly(A)+ RNA in the nucleus at the restrictive temperature. A total of 21 recessive mutants were isolated and classified into 16 complementat...
Topics
- Biological Transport
- Blotting, Northern
- Fungal Proteins
- Genetic Complementation Test
- In Situ Hybridization, Fluorescence
- Mutation
- Poly A
- Pyrrolidinones
- RNA Processing, Post-Transcriptional
- RNA, Fungal
- RNA, Messenger
- Saccharomyces cerevisiae