Article
The use of the RACE method to clone hybridoma cDNA when V region primers fail.
Journal of immunological methods - 12 Jul 1994
Ruberti F, Cattaneo A, Bradbury A
Abstract excerpt
The technique of V region PCR to clone antibody V regions from hybridomas has been extensively used. However, in addition to, or even instead of, cloning the V regions with the desired specificity, myeloma cell derived V regions, V regions which are the result of non-productive rearrangements, and also V regions which are productive but which do not recognise the antigen of interest, may be isolated. In this...
Topics
- Animals
- Base Sequence
- Binding Sites
- Cloning, Molecular
- DNA Primers
- DNA, Complementary
- Gene Amplification
- Hybridomas
- Immunoglobulin Variable Region
- Immunologic Techniques
- Mice