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Article

Rapid isolation of immunoglobulin variable genes from cell lysates of rat hybridomas by polymerase chain reaction.

Hybridoma - 1 Feb 1992

Kütemeier G, Harloff C, Mocikat R

Abstract excerpt

The isolation of the rearranged immunoglobulin genes from a hybridoma cell line, which is a prerequisite for the construction of a recombinant antibody, can easily be achieved by polymerase chain reaction. Here we demonstrate that this method, which was originally described for cloning murine immunoglobulin genes from cDNA, is also applicable for rat genes. We show that the procedure also works with crude cell...

Topics

Alleles
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Base Sequence
Cloning, Molecular
DNA
DNA-Directed DNA Polymerase
Hybridomas
Immunoglobulin Variable Region
Mice

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Polymerase Chain Reaction

Rats

Taq Polymerase

Source

Identifiers

PMID: 1737637
DOI: 10.1089/hyb.1992.11.23

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1737637-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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