Article
ATP synthase of yeast mitochondria. Characterization of subunit d and sequence analysis of the structural gene ATP7.
The Journal of biological chemistry - 5 Sept 1991
Norais N, Promé D, Velours J
Abstract excerpt
The subunit analogous to the d-subunit of ATP synthase from bovine heart mitochondria was isolated from the purified yeast enzyme. Partial protein sequences were determined by direct methods. From this information, two oligonucleotide probes were constructed and used for screening a DNA genomic bank of Saccharomyces cerevisiae. The sequence of yeast subunit d was deduced from the DNA sequence of ATP7 gene. Mature...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA, Fungal
- Electrophoresis, Polyacrylamide Gel
- Genes, Fungal
- Genomic Library
- Mass Spectrometry
- Mitochondria
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
