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Article

Accumulation of an artificial cell wall-binding lipase by Bacillus subtilis wprA and/or sigD mutants.

FEMS microbiology letters - 15 Jul 2000

Kobayashi G, Toida J, Akamatsu T, Yamamoto H, Shida T, Sekiguchi J

Abstract excerpt

A recombinant lipase, CWB-LipB, localized on the Bacillus subtilis cell surface and retaining lipase activity was unstable and not accumulated in a high yield. To improve the accumulation, we examined cell wall binding protease (wprA)- and/or sigma D (sigD)-deficient mutants, and also a NprE and AprA protease-deficient mutant as host strains. The nprE aprA mutation did not lead to a significant increase in the...

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PMID: 10913700
DOI: 10.1111/j.1574-6968.2000.tb09188.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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