Article
Interaction with arginine 597 of NADPH-cytochrome P-450 oxidoreductase is a primary source of the uniform binding energy used to discriminate between NADPH and NADH.
Biochemistry - 2 Nov 1993
Sem D S, Kasper C B
Abstract excerpt
Site-directed mutagenesis has been used in conjunction with pH and alternate substrate/inhibitor studies to characterize the interactions between NADPH-cytochrome P-450 oxidoreductase (P-450R) and the 2'-phosphate of NADP(H) that provide P-450R with its strong nicotinamide nucleotide specificity....
Topics
- Amino Acid Sequence
- Animals
- Arginine
- Base Sequence
- Escherichia coli
- Hydrogen-Ion Concentration
- Models, Chemical
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- NAD
- NADP
- NADPH-Ferrihemoprotein Reductase
- Protein Binding
- Rats
- Recombinant Proteins
- Structure-Activity Relationship
- Substrate Specificity