Article
Coenzyme binding during catalysis is beneficial for the stability of 4-hydroxyacetophenone monooxygenase.
The Journal of biological chemistry - 16 Sept 2005
van den Heuvel Robert H H, Tahallah Nora, Kamerbeek Nanne M, Fraaije Marco W, van Berkel Willem J H, Janssen Dick B, Heck Albert J R
Abstract excerpt
The NADPH-dependent dimeric flavoenzyme 4-hydroxyacetophenone monooxygenase (HAPMO) catalyzes Baeyer-Villiger oxidations of a wide range of ketones, thereby generating esters or lactones. In the current work, we probed HAPMO-coenzyme complexes present during the enzyme catalytic cycle with the aim to gain mechanistic insight. Moreover, we investigated the structural role of the nicotinamide coenzyme. For these...
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