Article
Mutations in the carboxy terminus of adeno-associated virus 2 capsid proteins affect viral infectivity: lack of an RGD integrin-binding motif.
The Journal of general virology - 1 Dec 1994
Ruffing M, Heid H, Kleinschmidt J A
Abstract excerpt
Using site-directed mutagenesis, we tested whether a potential integrin-binding site, (composed of the amino acids RGD) which is predicted in the adeno-associated virus 2 (AAV-2) capsid open reading frame (ORF), plays a role in the infectivity of AAV-2. Nucleotide sequencing of wild-type and mutant capsid protein-coding sequences, however, revealed discrepancies with the published sequence data at several...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Capsid
- DNA, Single-Stranded
- DNA, Viral
- Dependovirus
- Genes, Viral
- HeLa Cells
- Humans
- Integrins
- Molecular Sequence Data