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Article

Mutations on the external surfaces of adeno-associated virus type 2 capsids that affect transduction and neutralization.

Journal of virology - 1 Jan 2006

Lochrie Michael A, Tatsuno Gwen P, Christie Brian, McDonnell Jennifer Wellman, Zhou Shangzhen, Surosky Richard, Pierce Glenn F, Colosi Peter

Abstract excerpt

Mutations were made at 64 positions on the external surface of the adeno-associated virus type 2 (AAV-2) capsid in regions expected to bind antibodies. The 127 mutations included 57 single alanine substitutions, 41 single nonalanine substitutions, 27 multiple mutations, and 2 insertions. Mutants were assayed for capsid synthesis, heparin binding, in vitro transduction, and binding and neutralization by murine...

Topics

Alanine
Amino Acid Substitution
Animals
Antibodies, Monoclonal
Antibodies, Viral
Capsid
Dependovirus
Heparin
Humans
Immune Sera
Mice

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Models, Molecular

Mutation

Neutralization Tests

Transduction, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 16378984
DOI: 10.1128/JVI.80.2.821-834.2006
PMCID: PMC1346838

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