Article
Circadian clock mutants of cyanobacteria.
Science (New York, N.Y.) - 18 Nov 1994
Kondo T, Tsinoremas N F, Golden S S, Johnson C H, Kutsuna S, Ishiura M
Abstract excerpt
A diverse set of circadian clock mutants was isolated in a cyanobacterial strain that carries a bacterial luciferase reporter gene attached to a clock-controlled promoter. Among 150,000 clones of chemically mutagenized bioluminescent cells, 12 mutants were isolated that exhibit a broad spectrum of periods (between 16 and 60 hours), and 5 mutants were found that show a variety of unusual patterns, including...
Topics
- Circadian Rhythm
- Cloning, Molecular
- Cyanobacteria
- Darkness
- Genes, Bacterial
- Genetic Complementation Test
- Light
- Luminescent Measurements
- Mutagenesis
- Mutation
- Temperature