Article
Bacterial luciferase as a reporter of circadian gene expression in cyanobacteria.
Journal of bacteriology - 1 Apr 1995
Liu Y, Golden S S, Kondo T, Ishiura M, Johnson C H
Abstract excerpt
To allow continuous monitoring of the circadian clock in cyanobacteria, we previously created a reporter strain (AMC149) of Synechococcus sp. strain PCC 7942 in which the promoter of the psbAI gene was fused to Vibrio harveyi luciferase structural genes (luxAB) and integrated into the chromosome. Northern (RNA) hybridization and immunoblot analyses were performed to examine changes in abundance of the luxAB mRNA,...
Topics
- Circadian Rhythm
- Cyanobacteria
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genes, Reporter
- Luciferases
- Luminescent Measurements
- Mutation
- RNA, Bacterial
- RNA, Messenger
- Vibrio