Article
Large-scale screening of Arabidopsis circadian clock mutants by a high-throughput real-time bioluminescence monitoring system.
The Plant journal : for cell and molecular biology - 1 Oct 2004
Onai Kiyoshi, Okamoto Kazuhisa, Nishimoto Harumi, Morioka Chisato, Hirano Minako, Kami-Ike Nobunori, Ishiura Masahiro
Abstract excerpt
Using a high-throughput real-time bioluminescence monitoring system, we screened large numbers of Arabidopsis thaliana mutants for extensively altered circadian rhythms. We constructed reporter genes by fusing a promoter of an Arabidopsis flowering-time gene - either GIGANTEA (GI) or FLOWERING LOCUS T (FT) - to a modified firefly luciferase gene (LUC(+)), and we transferred the fusion gene (P(GI)::LUC(+) or...
Topics
- Arabidopsis
- Circadian Rhythm
- Flowers
- Genes, Reporter
- Luciferases
- Luminescent Measurements
- Monitoring, Physiologic
- Mutagenesis
- Mutation
