Article
Expression and targeting of a 47 kDa integral peroxisomal membrane protein of Candida boidinii in wild type and a peroxisome-deficient mutant of Hansenula polymorpha.
FEBS letters - 11 Jan 1993
Sulter G J, Waterham H R, Vrieling E G, Goodman J M, Harder W, Veenhuis M
Abstract excerpt
A 47 kDa integral peroxisomal membrane protein (PMP47) of Candida boidinii was expressed in wild type (WT) and a temperature-sensitive (Ts6) peroxisome-deficient (per) mutant of Hansenula polymorpha. The subcellular location of PMP47 appeared to be dependent on the level of expression. At low exp...
Topics
- Blotting, Western
- Candida
- Cloning, Molecular
- Fungal Proteins
- Immunohistochemistry
- Intracellular Membranes
- Membrane Proteins
- Microbodies
- Microscopy, Electron
- Mutation
- Pichia
- Plasmids