Article
Transfection of the ketogenic mitochondrial 3-hydroxy-3-methylglutaryl-coenzyme A synthase cDNA into Mev-1 cells corrects their auxotrophy for mevalonate.
The Journal of biological chemistry - 18 Nov 1994
Ortiz J A, Gil-Gómez G, Casaroli-Marano R P, Vilaró S, Hegardt F G, Haro D
Abstract excerpt
A somatic cell mutant of the Chinese hamster ovary (CHO)-K1 (called Mev-1), auxotrophic for mevalonate by virtue of a complete lack of detectable cytosolic 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase activity, was transfected with a plasmid containing the cDNA for ketogenic mitochond...
Topics
- Amino Acid Sequence
- Animals
- CHO Cells
- Cell Line
- Cholesterol
- Cricetinae
- DNA, Complementary
- Hydroxymethylglutaryl-CoA Synthase
- Ketones
- Magnesium Chloride
- Mevalonic Acid
- Microscopy, Immunoelectron
- Mitochondria
- Molecular Sequence Data
- Mutation
- Transfection