Article
Resonance Raman microprobe spectroscopy of rhodopsin mutants: effect of substitutions in the third transmembrane helix.
Biochemistry - 9 Jun 1992
Lin S W, Sakmar T P, Franke R R, Khorana H G, Mathies R A
Abstract excerpt
A microprobe system has been developed that can record Raman spectra from as little as 2 microL of solution containing only micrograms of biological pigments. The apparatus consists of a liquid nitrogen (l-N2)-cooled cold stage, an epi-illumination microscope, and a substractive-dispersion, double spectrograph coupled to a l-N2-cooled CCD detector. Experiments were performed on native bovine rhodopsin, rhodopsin...
Topics
- Alanine
- Animals
- Binding Sites
- Cattle
- Glutamates
- Glutamic Acid
- Glutamine
- Isomerism
- Models, Molecular
- Molecular Conformation
- Mutation
- Rhodopsin