Article
Reconstruction of the proteolytic pathway for use of beta-casein by Lactococcus lactis.
Molecular microbiology - 1 Mar 1998
Kunji E R, Fang G, Jeronimus-Stratingh C M, Bruins A P, Poolman B, Konings W N
Abstract excerpt
Amino acid auxotrophous bacteria such as Lactococcus lactis use proteins as a source of amino acids. For this process, they possess a complex proteolytic system to degrade the protein(s) and to transport the degradation products into the cell. We have been able to dissect the various steps of the pathway by deleting one or more genes encoding key enzymes/components of the system and using mass spectrometry to...
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