Article
Pseudomonas aeruginosa lasB1 mutants produce an elastase, substituted at active-site His-223, that is defective in activity, processing, and secretion.
Journal of bacteriology - 1 Jul 1993
McIver K S, Olson J C, Ohman D E
Abstract excerpt
Pseudomonas aeruginosa secretes elastase in a multistep process which begins with the synthesis of a preproelastase (53.6 kDa) encoded by lasB, is followed by processing to proelastase (51 kDa), and concludes with the rapid accumulation of mature elastase (33 kDa) in the extracellular environment...
Topics
- Alleles
- Binding Sites
- Cloning, Molecular
- DNA Mutational Analysis
- Enzyme Precursors
- Genetic Markers
- Histidine
- Mutagenesis, Insertional
- Pancreatic Elastase
- Protein Processing, Post-Translational
- Pseudomonas aeruginosa
- Restriction Mapping