Article
Variability of the E2/NS1 region of Swedish hepatitis C virus strains and its correlation to genotypes.
Scandinavian journal of infectious diseases - 1 Jan 1994
Johansson B, Yun Z B, Sönnerborg A
Abstract excerpt
Serum RNA was extracted from 5 Swedish patients infected with hepatitis C virus (HCV). The N-terminal part of the genomic region coding for the gp70 (E2/NS1), including the hypervariable domain of the E2 protein, was reverse transcribed and amplified by the polymerase chain reaction (PCR) using biotinylated primers. The amplicon was immobilized on magnetic polystyrene beads coated with streptavidine. Solid-state...
Topics
- Amino Acid Sequence
- Base Sequence
- Genetic Variation
- Genotype
- Hepacivirus
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- RNA, Viral
- Sequence Analysis, DNA
- Sweden
