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Article

Construction of a gyrA plasmid for genetic characterization of fluoroquinolone-resistant Staphylococcus aureus.

FEMS immunology and medical microbiology - 1 Jun 1994

Tankovic J, Duval J, Courvalin P

Abstract excerpt

We have constructed a gyrA trans-complementation plasmid, pAT512, by cloning the wild-type gyrA gene of Staphylococcus aureus into the expression vector pAT392. Introduction by electrotransformation of pAT512 into a high-level fluoroquinolone resistant mutant of S. aureus (ciprofloxacin MIC = 16 micrograms ml-1) having a gyrA mutation which results in a Ser-84 to Leu substitution, reduced the MICs of norfloxacin...

Topics

Anti-Infective Agents
Base Sequence
Ciprofloxacin
DNA Gyrase
DNA Probes
DNA Topoisomerases, Type II
Drug Resistance, Microbial
Fluoroquinolones
Molecular Sequence Data
Mutation
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Staphylococcus aureus

Source

Identifiers

PMID: 7920462
DOI: 10.1111/j.1574-695X.1994.tb00471.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7920462-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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