Article
Mutation of His-105 in the beta 1 subunit yields a nitric oxide-insensitive form of soluble guanylyl cyclase.
Proceedings of the National Academy of Sciences of the United States of America - 29 Mar 1994
Wedel B, Humbert P, Harteneck C, Foerster J, Malkewitz J, Böhme E, Schultz G, Koesling D
Abstract excerpt
Soluble guanylyl cyclase [GTP pyrophosphate-lyase (cyclizing); EC 4.6.1.2] is a hemoprotein that exists as a heterodimer; the heme moiety has been proposed to bind nitric oxide, resulting in a dramatic activation of the enzyme. Mutation of six conserved His residues reduced but did not abolish nitric oxide stimulation whereas a change of His-105 to Phe in the beta 1 subunit yielded a heterodimer that retained...
Topics
- Amino Acid Sequence
- Animals
- Cyclic GMP
- Enzyme Activation
- Glutathione
- Guanosine Triphosphate
- Guanylate Cyclase
- Heme
- Histidine
- Molecular Sequence Data
- Mutation