Article
Identification by mutational analysis of four critical residues in the molybdenum cofactor domain of eukaryotic nitrate reductase.
FEBS letters - 21 Aug 1995
Meyer C, Gonneau M, Caboche M, Rouzé P
Abstract excerpt
The nucleotide sequence of the nitrate reductase (NR) molybdenum cofactor (MoCo) domain was determined in four Nicotiana plumbaginifolia mutants affected in the NR apoenzyme gene. In each case, missense mutations were found in the MoCo domain which affected amino acids that were conserved not onl...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromatography, Gel
- Coenzymes
- DNA Mutational Analysis
- DNA, Complementary
- Metalloproteins
- Molecular Sequence Data
- Molybdenum Cofactors
- Mutation
- NAD
- Nitrate Reductase
- Nitrate Reductases
- Plants, Toxic
- Protein Conformation
- Pteridines
- Nicotiana