Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Sequential multiplex amplification (SMA) of genetic loci: a method for recovering template DNA for subsequent analyses of additional loci.

International journal of legal medicine - 1 Jan 1994

Lorente M, Lorente J A, Wilson M R, Budowle B, Villanueva E

Abstract excerpt

A method called Sequential Multiplex Amplification (SMA) has been developed whereby a limited amount of DNA extracted from a sample can be reutilized for several single polymerase chain reaction (PCR) amplifications. The method involves recovery of genomic template DNA by microfiltration of PCR-amplified samples. Up to 5 different loci have been typed, each in a single system PCR-based assay, beginning with a...

Topics

Base Sequence
DNA
Forensic Medicine
Genotype
Humans
Molecular Sequence Data
Nucleic Acid Amplification Techniques
Polymerase Chain Reaction
Templates, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 7893612
DOI: 10.1007/BF01225604

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7893612-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Sequential multiplex amplification (SMA) of genetic loci: a method for recovering template DNA for subsequent analyses of additional loci.PMID 7893612Read ↗

Multiplex universal genotyping using a modified ARMS-PCR protocol.Semantic proximity Read ↗

Multiplex genotype determination at a large number of gene loci.Semantic proximity Read ↗

AmpFlSTR profiler Plus short tandem repeat DNA analysis of casework samples, mixture samples, and nonhuman DNA samples amplified under reduced PCR volume conditions (25 microL).Semantic proximity Read ↗

Interpretation guidelines for mixed-STR multilocus electrophoretic profiles.Semantic proximity Read ↗

A simple protocol to improve touch DNA analysis using direct STR amplification.Semantic proximity Read ↗

SASA: a simplified, reliable method for allele-specific amplification of polymorphic sites.Semantic proximity Read ↗

Analysis and interpretation of mixed profiles generated by 34 cycle SGM Plus(®) amplification.Semantic proximity Read ↗

Decreasing amplification bias associated with multiple displacement amplification and short tandem repeat genotyping.Semantic proximity Read ↗

A single multiplex PCR and SNaPshot minisequencing reaction of 42 SNPs to classify admixture populations into mitochondrial DNA haplogroups.Semantic proximity Read ↗

Molecular crowding increases the amplification success of multiple displacement amplification and short tandem repeat genotyping.Semantic proximity Read ↗

Allele-specific polymerase chain reaction: a method for amplification and sequence determination of a single component among a mixture of sequence variants.Semantic proximity Read ↗

Modeling one complete versus triplicate analyses in low template DNA typing.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.