Article
Characterization of wild-type and amyotrophic lateral sclerosis-related mutant Cu,Zn-superoxide dismutases overproduced in baculovirus-infected insect cells.
Journal of neurochemistry - 1 Apr 1995
Fujii J, Myint T, Seo H G, Kayanoki Y, Ikeda Y, Taniguchi N
Abstract excerpt
We describe the use of a baculovirus expression system to overproduce human Cu,Zn-superoxide dismutase (SOD). Spodoptera frugiperda (Sf21) insect cells infected with a baculovirus carrying the Cu,Zn-SOD cDNA synthesized a large amount of Cu,Zn-SOD apoprotein in the conventional medium. The SOD ac...
Topics
- Amyotrophic Lateral Sclerosis
- Animals
- Apoenzymes
- Bacterial Infections
- Baculoviridae
- Base Sequence
- Cell Line
- Chromatography, Gel
- Chromatography, Ion Exchange
- Copper
- Ions
- Molecular Probes
- Molecular Sequence Data
- Mutation
- Spodoptera
- Superoxide Dismutase