Article
Molecular cloning and expression, in both COS-1 cells and S. cerevisiae, of a human cytosolic type-IVA, cyclic AMP specific phosphodiesterase (hPDE-IVA-h6.1).
Cellular signalling - 1 Sept 1994
Sullivan M, Egerton M, Shakur Y, Marquardsen A, Houslay M D
Abstract excerpt
Screening a human T lymphocyte cDNA library with a phosphodiesterase (PDE) specific probe resulted in the isolation of two overlapping cDNA clones, h2.2 and h6.1, that encode a type IV, rolipram inhibited cAMP-specific PDE. Clones h2.2 and h6.1 were 1015 bp and 2288 bp in length, respectively, an...
Topics
- 3',5'-Cyclic-AMP Phosphodiesterases
- Amino Acid Sequence
- Animals
- Base Sequence
- Cattle
- Cell Line
- Cell Line, Transformed
- Cloning, Molecular
- Cytosol
- DNA, Complementary
- Gene Expression
- Haplorhini