Article
Isolation and characterization of cDNAs encoding PDE5A, a human cGMP-binding, cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase.
Gene - 17 Aug 1998
Loughney K, Hill T R, Florio V A, Uher L, Rosman G J, Wolda S L, Jones B A, Howard M L, McAllister-Lucas L M, Sonnenburg W K, Francis S H, Corbin J D, Beavo J A, Ferguson K
Abstract excerpt
Human cGMP-binding, cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase (PDE5A) cDNAs were isolated. A 3.1-kb composite DNA sequence assembled from overlapping cDNAs encodes an 875-amino-acid protein with a predicted molecular mass of 100012 Da (PDE5A1). Extracts prepared from yeast expressing human PDE5A1 hydrolyzed cGMP. This activity was inhibited by the selective PDE5 inhibitors zaprinast and DMPPO. PDE5A...
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