Article
Identification of new and unusual rev and nef transcripts expressed by an HIV type 1 primary isolate.
AIDS research and human retroviruses - 1 Jul 2013
Vega Yolanda, Delgado Elena, Carrera Cristina, Nebreda Paloma, Fernández-García Aurora, Cuevas María Teresa, Pérez-Álvarez Lucía, Thomson Michael M
Abstract excerpt
We analyzed RNA splice site usage in three HIV-1 subtype B primary isolates through reverse transcriptase polymerase chain reaction (RT-PCR) amplification of spliced RNAs using a fluorescently labeled primer, with computerized size determination and quantification of PCR products, which were also identified by clone sequencing. In one isolate, P2149-3, unusual and unreported spliced transcripts were detected....
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