Article
The end of the cob operon: evidence that the last gene (cobT) catalyzes synthesis of the lower ligand of vitamin B12, dimethylbenzimidazole.
Journal of bacteriology - 1 Mar 1995
Chen P, Ailion M, Weyand N, Roth J
Abstract excerpt
The cob operon of Salmonella typhimurium includes 20 genes devoted to the synthesis of adenosyl-cobalamin (coenzyme B12). Mutants with lesions in the promoter-distal end of the operon synthesize vitamin B12 only if provided with 5,6-dimethylbenzimidazole (DMB), the lower ligand of vitamin B12. In...
Topics
- Amino Acid Sequence
- Base Sequence
- Benzimidazoles
- Cloning, Molecular
- Cobamides
- Genes, Bacterial
- Genetic Complementation Test
- Molecular Sequence Data
- Mutagenesis, Insertional
- Open Reading Frames
- Operon
- Pentosyltransferases
- Phenotype
- RNA, Transfer, Ser
- Salmonella typhimurium
- Sequence Homology, Amino Acid