Article
YBR1012 an essential gene from S. cerevisiae: construction of an RNA antisense conditional allele and isolation of a multicopy suppressor.
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie - 1 Jul 1994
Nasr F, Bécam A M, Slonimski P P, Herbert C J
Abstract excerpt
The gene YBR1012 was identified during the systematic sequencing of chromosome II of the yeast Saccharomyces cerevisiae. We have inactivated the gene and shown that it is essential for cellular viability. Using antisense RNA technology we have constructed a conditional allele, expression of the antisense RNA strongly inhibits growth. To our knowledge this is the first successful use of antisense RNA technology in...
Topics
- Alleles
- Genes, Fungal
- Genes, Suppressor
- Molecular Sequence Data
- RNA, Antisense
- RNA, Fungal
- Saccharomyces cerevisiae
- Schizosaccharomyces
- Sequence Alignment