Article
Establishment of a human melanoma cell line lacking p53 expression and spontaneously metastasizing in nude mice.
Anticancer research - 1 Jan 2000
Rauth S, Kichina J, Green A, Bratescu L, Das Gupta T K
Abstract excerpt
Nine human melanoma cell lines established in our laboratory were analyzed for p53 gene expression and their tumorigenic and metastatic potential in nude mice. Northern blot analyses showed that five of the cell lines (55%) had either complete loss or low levels of p53 transcripts. Immunocytochemical analysis for p53 protein expression agreed with mRNA analysis results. Nucleotide sequencing showed no mutations...
Topics
- Animals
- Base Sequence
- Blotting, Northern
- Cell Division
- Cell Line
- DNA, Neoplasm
- Exons
- Gene Expression
- Genes, p53
- Humans
- Immunohistochemistry
- Melanoma