Article
Phosphatase-negative mutants of Legionella pneumophila and their behavior in mammalian cell infection.
Microbial pathogenesis - 1 Jul 1994
Kim M J, Rogers J E, Hurley M C, Engleberg N C
Abstract excerpt
Microbial phosphatases are known or suspected to play a role in the pathogenesis of several intracellular pathogens, including Legionella micdadei. Legionella pneumophila also possess phosphatase activities, but their possible roles in cellular infection are unknown. We generated mutants of a serogroup 1 isolate of L. pneumophila that lack the major phosphatase. Isolation of a Pho- mutant after random mutagenesis...
Topics
- Alkaline Phosphatase
- Cell Line
- Cloning, Molecular
- DNA Transposable Elements
- Humans
- Legionella pneumophila
- Mutation
- Phosphates
- Transformation, Bacterial