Article
Long-term repopulation of irradiated mice with limiting numbers of purified hematopoietic stem cells: in vivo expansion of stem cell phenotype but not function.
Blood - 15 Feb 1995
Spangrude G J, Brooks D M, Tumas D B
Abstract excerpt
Hematopoietic stem cells were isolated from normal adult mouse bone marrow based on surface antigen expression (Thy-1.1(low)Lin(neg)Ly-6A/E+) and further selected for low retention of rhodamine 123. This population of cells (Rh-123low) could mediate radioprotection and long-term (greater than 12...
Topics
- Animals
- Antigens, Surface
- B-Lymphocytes
- Bone Marrow
- Cell Separation
- Cells, Cultured
- Cesium Radioisotopes
- Chimera
- Flow Cytometry
- Fluorescent Dyes
- Granulocytes
- Hematopoietic Stem Cell Transplantation
- Hematopoietic Stem Cells
- Mice
- Mice, Inbred C57BL
- Mice, Inbred Strains
- Monocytes
- Phenotype