Article
Probing of the location of the allosteric site on m1 muscarinic receptors by site-directed mutagenesis.
Molecular pharmacology - 1 Jan 1995
Matsui H, Lazareno S, Birdsall N J
Abstract excerpt
In an attempt to locate the allosteric site on muscarinic receptors to which gallamine binds, 21 residues in the putative external loops and loop/transmembrane helix interfaces have been mutated to alanine. These residues are conserved in mammalian m1-m5 receptors. All mutant receptors can be exp...
Topics
- Acetylcholine
- Alanine
- Allosteric Regulation
- Animals
- Base Sequence
- Binding Sites
- Binding, Competitive
- Gallamine Triethiodide
- Kinetics
- Molecular Sequence Data
- Muscarinic Antagonists
- Mutagenesis, Site-Directed