Article
Probing of the location of the allosteric site on m1 muscarinic receptors by site-directed mutagenesis.
Molecular pharmacology - 1 Jan 1995
Matsui H, Lazareno S, Birdsall N J
Abstract excerpt
In an attempt to locate the allosteric site on muscarinic receptors to which gallamine binds, 21 residues in the putative external loops and loop/transmembrane helix interfaces have been mutated to alanine. These residues are conserved in mammalian m1-m5 receptors. All mutant receptors can be expressed in COS-7 cells at high levels and appear to be functional, in that acetylcholine binding is sensitive to GTP....
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