Article
Subunit interface mutants of rabbit muscle aldolase form active dimers.
Protein science : a publication of the Protein Society - 1 Sept 1994
Beernink P T, Tolan D R
Abstract excerpt
We report the construction of subunit interface mutants of rabbit muscle aldolase A with altered quaternary structure. A mutation has been described that causes nonspherocytic hemolytic anemia and produces a thermolabile aldolase (Kishi H et al., 1987, Proc Natl Acad Sci USA 84:8623-8627). The di...
Topics
- Anemia, Hemolytic
- Animals
- Base Sequence
- Centrifugation, Density Gradient
- Circular Dichroism
- Cross-Linking Reagents
- Enzyme Stability
- Escherichia coli
- Fructose-Bisphosphate Aldolase
- Humans
- Molecular Sequence Data
- Muscles
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation
- Protein Denaturation
- Rabbits
- Recombinant Proteins