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Article

Biophysical characterization of the dimer and tetramer interface interactions of the human cytosolic malic enzyme.

PloS one - 1 Jan 2012

Murugan Sujithkumar, Hung Hui-Chih

Abstract excerpt

The cytosolic NADP(+)-dependent malic enzyme (c-NADP-ME) has a dimer-dimer quaternary structure in which the dimer interface associates more tightly than the tetramer interface. In this study, the urea-induced unfolding process of the c-NADP-ME interface mutants was monitored using fluorescence and circular dichroism spectroscopy, analytical ultracentrifugation and enzyme activities. Here, we demonstrate the...

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Identifiers

PMID: 23284632
DOI: 10.1371/journal.pone.0050143
PMCID: PMC3528742

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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